• Email us: tamechimportandexport@gmail.com

ELISA Kits for T. Parva

ELISA Kits for T. Parva

ELISA Kits for T. Parva

SEND INQUIRY

Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number Q9NVD7
Gene Symbols MXRA2|PARVA
Gene Id 55742
Protein Name / Synonyms Alpha-parvin (Actopaxin) (CH-ILKBP) (Calponin-like integrin-linked kinase-binding protein) (Matrix-remodeling-associated protein 2)
Quantitative/Semi-Quantitative Quantitative
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Note This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 – 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech’s ELISA quality control tests, and will thereafter have a typical 1 – 2 day lead time for orders placed.
Estimated Lead Time 4-7 weeks
Storage -20°C

Risk-Free Guarantee

We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Related products

ABOUT US

ABOUT US

OUR CONTACTS

  • 198 / Nueng Ratchadaphisek Road,Huai Khwang Subdi Bangkok, Thailand
  • tamechimportandexport@gmail.com
  • Tel/whatsapp: +66 80 527 4303

Copyright © 2025, ALL RIGHTS RESERVED