Ethidium bromide is a fluorescent dye commonly used in molecular biology to visualize nucleic acids (DNA and RNA) in gel electrophoresis. It intercalates into the DNA double helix, causing it to fluoresce under UV light.
While it is a valuable tool for DNA analysis, ethidium bromide is a potent mutagen and must be handled with strict safety precautions, including the use of gloves and proper disposal methods.
Researchers are increasingly turning to safer alternatives, such as SYBR Green, but ethidium bromide remains a staple in many labs due to its affordability and effectiveness.